Path Category
Loading Path Category from the AllPath API…
ALLPATHFoundationALLPATHFoundationPath Category
Loading Path Category from the AllPath API…
Path Category
Guided learning journeys that build knowledge step by step.
category · Learning · slug · learning · 7817 Paths
7817 Paths · page 594 / 782
This learning path guides graduate students in gene regulation labs through the principles and practice of Electrophoretic Mobility Shift Assay (EMSA). Starting with fundamental protein-DNA binding concepts, it progresses through probe preparation, binding reactions, native gel electrophoresis, and advanced techniques like super-shift and competition assays. The path emphasizes hands-on skills and data interpretation for studying specific protein-DNA interactions.
This advanced graduate-level path equips molecular biology researchers with the knowledge and practical skills to design, deliver, and validate RNA interference (RNAi) experiments for gene silencing. It covers the mechanistic basis of RNAi, siRNA and shRNA design principles, transfection and delivery methods, knockdown validation, and functional assays, culminating in a comprehensive understanding of RNAi as a research tool.
This graduate-level path equips genetic engineering students with the knowledge to design and apply CRISPR systems for gene editing. It covers the underlying mechanism, sgRNA design, Cas9 variants, delivery methods, gene knock-out/knock-in strategies, and off-target considerations, grounded in basic cloning and molecular biology prerequisites.
This learning path guides biochemistry and molecular biology researchers through the essential knowledge and laboratory skills required to express and purify recombinant proteins. It covers expression systems (E. coli and yeast), protein purification techniques (affinity chromatography and gel filtration), and analytical methods (SDS-PAGE and Western blot), building from basic protein knowledge to advanced applications.
This learning path guides molecular biology researchers through the fundamental concepts and laboratory techniques required to accurately measure gene expression levels. It covers RNA biology basics, sample preparation, and three core measurement methods—Northern blot, qPCR, and RNA-seq—culminating in differential expression analysis. The path emphasizes practical skills and data interpretation, preparing learners to choose and apply appropriate methods in their research.
This advanced graduate-level learning path covers the principles, applications, and data analysis of major DNA sequencing technologies, from Sanger sequencing to next-generation and third-generation platforms. Learners will build a foundation in molecular biology and genomics, then explore each technology's methodology, advantages, limitations, and real-world applications, culminating in hands-on data analysis skills.
This learning path guides molecular biology students through the essential techniques for cloning DNA into plasmids, starting with basic molecular biology concepts and progressing through restriction digestion, ligation, transformation, blue-white screening, and plasmid purification. Each step builds on the previous one, ensuring a solid understanding of the cloning workflow.
This learning path guides lab students and technicians from foundational molecular biology to advanced PCR techniques. It covers the principles of PCR, primer design, optimization, and quantitative PCR (qPCR) using SYBR Green and TaqMan probes, including melt curve analysis. The path emphasizes hands-on skills and troubleshooting for real-world laboratory applications.
This learning path introduces biotechnology students to the core molecular tools and techniques used to create genetically modified organisms (GMOs). It covers the central dogma, restriction enzymes, vectors, transformation, transgenics, and the biosafety and regulatory frameworks that govern GMO applications. Designed for intermediate learners, the path progresses from foundational concepts to applied techniques and responsible use.
This learning path guides forensic science students from basic genetics through the molecular techniques used in forensic analysis, including DNA fingerprinting, STR analysis, mitochondrial DNA, population genetics, and contamination control. It builds a solid foundation in DNA structure and inheritance before progressing to specialized forensic applications.