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Learning
Membrane Excitability and Ion Flux in Neurons → Designing a Complete In Vivo Optogenetics Experiment
A structured path for learners who want to understand and apply optogenetics in living animals. It begins with the neurophysiology and photobiology that make light-driven control possible, builds through the molecular biology of channelrhodopsin and halorhodopsin, covers opsin selection, gene delivery, and hardware, and culminates in designing, executing, and interpreting an in vivo optogenetics experiment with appropriate controls. Emphasis is placed on why each opsin behaves the way it does, how to choose among available tools, and how to avoid the common artifacts of light delivery, heating, and expression variability. Learners finish able to read primary optogenetics literature critically and to plan a defensible in vivo manipulation experiment.
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16 steps · 4 stages. Click any step to inspect it and see it on the Path Map.
Curated materials referenced by this learning path.
Review by Deisseroth surveying opsin development, delivery, and in vivo applications; useful for framing the field and for the opsin variant and strategy nodes.
Open resource →Review covering channelrhodopsin and halorhodopsin families, their biophysics, and early in vivo use.
Open resource →Boyden et al. 2005, the foundational demonstration of channelrhodopsin-2 for millisecond-scale optical control of neurons.
Open resource →Compares halorhodopsin and archaerhodopsin silencing and their presynaptic side effects; directly informs the inhibition strategy and controls nodes.
Open resource →Systematic side-by-side comparison of opsin kinetics, sensitivity, and photocurrents; the key reference for variant selection.
Open resource →Review of practical in vivo optogenetics, including targeting strategies, light delivery, and controls.
Open resource →