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光在生物组织中的传播与衰减 → 设计一个可执行的光遗传学实验方案
本路径面向希望系统理解光遗传学(optogenetics)基本原理与常见光敏蛋白的入门学习者。路径从光与生物组织相互作用、神经元电活动基础出发,逐步建立光遗传学的核心概念:光敏蛋白的分类与工作机制、基因递送与表达策略、光纤与光源植入、光刺激参数与实验设计、行为学读出与数据分析,最后以设计一个可执行的光遗传学实验方案为终点。完成本路径后,学习者能够解释光敏蛋白如何把光信号转化为离子流或生化信号,能够根据实验目标选择合适的光敏蛋白与递送方式,并能够规划包含对照、光照参数和读出指标的基础实验。
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